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一步法人血管舒張劑激活磷蛋白(VASP)酶聯免疫吸附檢測試劑盒

ELK025ES

規格：	價格：
64T	￥2240.00
112T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Human VASP(Vasodilator Stimulated Phosphoprotein) ELISA Kit
Reactivity:	Human
Alternative Names:	Vasodilator-stimulated phosphoprotein
Assay Type:	Sandwich
Sensitivity:	17.5 pg/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Signal transduction;
Uniprot ID:	P50552
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VASP, and the Human VASP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VASP. After TMB substrate solution is added, only those wells that contain Human VASP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VASP in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.201	2.048
5.00	1.587	1.434
2.50	1.073	0.920
1.25	0.757	0.604
0.63	0.468	0.315
0.32	0.397	0.244
0.16	0.208	0.055
0.00	0.153	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VASP and the recovery rates were calculated by comparing the measured value to the expected amount of VASP in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VASP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.