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一步法人血管內皮生長因子165(VEGF165)酶聯免疫吸附檢測試劑盒

ELK027ES

規格：	價格：
64T	￥2240.00
112T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Human VEGF165(Vascular Endothelial Growth Factor 165) ELISA Kit
Reactivity:	Human
Alternative Names:	VEGF165
Assay Type:	Sandwich
Sensitivity:	15 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Cytokine;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165, and the Human VEGF165 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGF165. After TMB substrate solution is added, only those wells that contain Human VEGF165 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGF165 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.126	2.033
1000.00	1.731	1.638
500.00	1.114	1.021
250.00	0.855	0.762
125.00	0.532	0.439
62.50	0.335	0.242
31.25	0.226	0.133
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VEGF165 and the recovery rates were calculated by comparing the measured value to the expected amount of VEGF165 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-108%	101%
EDTA plasma(n=5)	83-97%	90%
Heparin plasma(n=5)	80-97%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF165 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.