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一步法人干擾素γ誘導單核因子(MIg)酶聯免疫吸附檢測試劑盒

ELK032ES

規格：	價格：
64T	￥1960.00
112T	￥2800.00

下載說明 ①

Overview

Product name:	EasyStep Human MIg(Monokine Induced By Interferon Gamma) ELISA Kit
Reactivity:	Human
Alternative Names:	CXCL9; CMK; Humig; MI-G; SCYB9; Crg-10; Chemokine (C-X-C motif) ligand 9; Small-inducible cytokine B9; Gamma-interferon-induced monokine
Assay Type:	Sandwich
Sensitivity:	15.7 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.2-5000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Cytokine;Infection immunity;
Uniprot ID:	Q07325
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIg, and the Human MIg standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MIg. After TMB substrate solution is added, only those wells that contain Human MIg and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIg in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.258	2.176
2500.00	1.700	1.618
1250.00	1.279	1.197
625.00	0.828	0.746
312.50	0.555	0.473
156.25	0.324	0.242
78.13	0.187	0.105
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MIg and the recovery rates were calculated by comparing the measured value to the expected amount of MIg in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	78-90%	84%
Heparin plasma(n=5)	89-107%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIg and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.