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一步法人氨基端前腦鈉素(NT-ProBNP)酶聯免疫吸附檢測試劑盒

ELK035ES

規格：	價格：
64T	￥1960.00
112T	￥2800.00

下載說明 ①

Overview

Product name:	EasyStep Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity:	Human
Alternative Names:	NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type:	Sandwich
Sensitivity:	0.94 ng/mL
Standard:	60 ng/mL
Detection Range:	0.94-60 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
60.00	2.207	2.106
30.00	1.643	1.542
15.00	1.251	1.150
7.50	0.847	0.746
3.75	0.625	0.524
1.88	0.301	0.200
0.94	0.288	0.187
0.00	0.101	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.

Matrix	Recovery range	Average
serum(n=5)	90-103%	96%
EDTA plasma(n=5)	81-96%	88%
Heparin plasma(n=5)	78-94%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.