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一步法人血管細胞粘附分子1(VCAM1)酶聯免疫吸附檢測試劑盒

ELK043ES

規格：	價格：
64T	￥1960.00
112T	￥2800.00

下載說明 ①

Overview

Product name:	EasyStep Human VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit
Reactivity:	Human
Alternative Names:	CD106; INCAM-100; L1CAM
Assay Type:	Sandwich
Sensitivity:	0.87 ng/mL
Standard:	80 ng/mL
Detection Range:	1.25-80 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	CD & Adhesion molecule;Tumor immunity;Infection immunity;
Uniprot ID:	P19320
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCAM1, and the Human VCAM1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VCAM1. After TMB substrate solution is added, only those wells that contain Human VCAM1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
80.00	1.948	1.855
40.00	1.642	1.549
20.00	1.139	1.046
10.00	0.797	0.704
5.00	0.527	0.434
2.50	0.319	0.226
1.25	0.227	0.134
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VCAM1 and the recovery rates were calculated by comparing the measured value to the expected amount of VCAM1 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-99%	92%
EDTA plasma(n=5)	78-92%	85%
Heparin plasma(n=5)	78-90%	84%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VCAM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.