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一步法人生長分化因子15(GDF15)酶聯免疫吸附檢測試劑盒

ELK044ES

規格：	價格：
64T	￥2240.00
112T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Human GDF15(Growth Differentiation Factor 15) ELISA Kit
Reactivity:	Human
Alternative Names:	PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor
Assay Type:	Sandwich
Sensitivity:	5.22 pg/mL
Standard:	3000 pg/mL
Detection Range:	46.88-3000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Cytokine;Apoptosis;Tumor immunity;Infection immunity;
Uniprot ID:	Q99988
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF15, and the Human GDF15 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human GDF15. After TMB substrate solution is added, only those wells that contain Human GDF15 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
3000.00	2.401	2.329
1500.00	1.472	1.400
750.00	1.144	1.072
375.00	0.795	0.723
187.50	0.595	0.523
93.75	0.305	0.233
46.88	0.190	0.118
0.00	0.072	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GDF15 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF15 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	94%
EDTA plasma(n=5)	84-95%	88%
Heparin plasma(n=5)	82-97%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDF15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.