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一步法大鼠25-羥基維生素D(25-OH-D)酶聯免疫吸附檢測試劑盒

ELK053ES

規格：	價格：
64T	￥2660.00
112T	￥3800.00

下載說明 ①

Overview

Product name:	EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Reactivity:	Rat
Alternative Names:	25 Hydroxy Vitamin D;
Assay Type:	Competitive Inhibition
Sensitivity:	7.7 ng/mL
Standard:	5000 ng/mL
Detection Range:	78.13-5000 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
5000.00	0.189
2500.00	0.397
1250.00	0.583
625.00	0.833
312.50	1.157
156.25	1.583
78.13	1.715
0.00	2.211

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 25-OH-D and the recovery rates were calculated by comparing the measured value to the expected amount of 25-OH-D in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	91-105%	98%
Heparin plasma(n=5)	95-107%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.