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一步法大鼠血管內皮生長因子受體2(VEGFR2)酶聯免疫吸附檢測試劑盒

ELK083ES

規格：	價格：
64T	￥2240.00
112T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Rat VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
Reactivity:	Rat
Alternative Names:	CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1
Assay Type:	Sandwich
Sensitivity:	157.5 pg/mL
Standard:	50 ng/mL
Detection Range:	0.78-50 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Tumor immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFR2, and the Rat VEGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat VEGFR2. After TMB substrate solution is added, only those wells that contain Rat VEGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
50.00	2.168	2.074
25.00	1.657	1.563
12.50	1.116	1.022
6.25	0.895	0.801
3.13	0.428	0.334
1.57	0.423	0.329
0.79	0.248	0.154
0.00	0.094	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VEGFR2 and the recovery rates were calculated by comparing the measured value to the expected amount of VEGFR2 in samples.

Matrix	Recovery range	Average
serum(n=5)	79-93%	86%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	85-95%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.