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一步法人白介素2受體a鏈(IL-2Ra/CD25)酶聯免疫吸附檢測試劑盒

ELK087ES

規格：	價格：
64T	￥2240.00
112T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Reactivity:	Human
Alternative Names:	IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type:	Sandwich
Sensitivity:	37.66 pg/mL
Standard:	6000 pg/mL
Detection Range:	93.75-6000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
6000.00	2.189	2.088
3000.00	1.582	1.481
1500.00	1.243	1.142
750.00	0.935	0.834
375.00	0.503	0.402
187.50	0.285	0.184
93.75	0.219	0.118
0.00	0.101	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL-2Ra/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2Ra/CD25 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-97%	89%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2Ra/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.