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人內分泌腺來源血管內皮生長因子(EG-VEGF)酶聯免疫吸附檢測試劑盒

ELK1008

規格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview

Product name:	Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit
Reactivity:	Human
Alternative Names:	PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine
Assay Type:	Sandwich
Sensitivity:	6.6 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;
Uniprot ID:	P58294
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.266	2.083
500.00	1.803	1.620
250.00	1.386	1.203
125.00	0.971	0.788
62.50	0.630	0.447
31.25	0.414	0.231
15.63	0.252	0.069
0.00	0.183	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant EG-VEGF and the recovery rates were calculated by comparing the measured value to the expected amount of EG-VEGF in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	79-93%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EG-VEGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.