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下載說明 ①Overview 文獻
日期：2024-06-14
IF：2.6標準曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 400.00 | 0.199 | |
| 200.00 | 0.352 | |
| 100.00 | 0.577 | |
| 50.00 | 0.895 | |
| 25.00 | 1.139 | |
| 12.50 | 1.578 | |
| 6.25 | 1.789 | |
| 0.00 | 2.115 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant 5-HIAA and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HIAA in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-105% | 97% |
| EDTA plasma(n=5) | 85-97% | 91% |
| Heparin plasma(n=5) | 92-105% | 93% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HIAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 89-97% | 85-94% | 98-105% | 92-101% |
| EDTA plasma(n=5) | 92-101% | 87-98% | 85-92% | 79-96% |
| Heparin plasma(n=5) | 89-102% | 88-97% | 88-104% | 97-108% |
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