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斑馬魚白介素17A(IL17A)酶聯(lián)免疫吸附檢測試劑盒

ELK8510

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Zebrafish IL17A(Interleukin 17A) ELISA Kit
Reactivity:	Zebrafish
Alternative Names:	IL17; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
Assay Type:	Sandwich
Sensitivity:	2.58 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish IL17A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish IL17A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish IL17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish IL17A in the samples is then determined by comparing the OD of the samples to the standard curve.

Naunyn-Schmiedeberg's Archives of Pharmacology

Protective effects of diacerein against quinolinic acid–induced Huntington’s disease–like symptoms in adult zebrafish by targeting GSK-3β signalling

日期：2025-04-17 IF：

標(biāo)準曲線

Concentration (pg/mL)	OD	Corrected OD
500.00	2.103	2.008
250.00	1.593	1.498
125.00	1.073	0.978
62.50	0.866	0.771
31.25	0.585	0.490
15.63	0.297	0.202
7.82	0.230	0.135
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL17A and the recovery rates were calculated by comparing the measured value to the expected amount of IL17A in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	78-95%	86%
Heparin plasma(n=5)	80-96%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL17A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.