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Overview

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  IP antibody use：3ug Flag Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with C-Myc-tag fusion protein 3、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4、IP (transfected 293+anti- C-Myc mAb+ P
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  1ug C-Myc fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000
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  IF analysis of 293 cells transfected with a C-Myc-tag protein, using ELKbio anti-C-MycTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-C-Myc)